Abstract :

Post-translational Modifications (PTMs) Are Major Players In The Regulation Of Protein Function, And They Also Affect Signal Transduction, Gene Expression, And Cellular Homeostasis Among Others. The Classic Techniques Used To Analyze PTMs Are Often Imprecise And May Not Provide An Exact Picture Of What Happens Under Physiological Conditions. On The Other Hand, Chemical Biology Approaches, Including Site-specific Incorporation Of PTMs, Are More Precise And Give Rise To Detailed Analysis. Among The Mentioned Approaches, Native Chemical Ligation, Expressed Protein Ligation, And Bioorthogonal Chemistry Are Mentioned As The Ones That Make Possible The Synthesis Of Proteins With Defined Modifications Which Consequently Allows The Study Of Their Functional Roles. The Application Of These Methods Allows Revealing The Effects Of Single PTMs On Protein Structure And Function And, Thereby, The Cellular Processes And Disease Mechanisms Insights Gained. The Paper At Hand Discusses Present-day Chemical Techniques For PTM Incorporation, Their Functional Proteomics Applications, And Newly Emerging Directions In The PTM-mediated Regulation Study. The Knowledge Of PTM Through Chemical Approaches Is Very Important Not Only For The Enhancement Of Our Understanding Of The Cellular Regulation But Also For The Discovery Of New Targeted Therapeutics. Keywords: Chemo Selective Ligation; Post-translational Modification; Protein Glycosylation; Protein Modification; Synthetic Proteins.

Published:

03-2-2024

Issue:

Vol. 24 No. 2 (2024)

Page Nos:

274-279

Section:

Articles

License:

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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